The mobile phase is the liquid moving through the pores in the paper. Before I delve into the analysis of the unknowns, why I picked them, and why I think I was wrong, I will explain why the two solvents my group used were the best. This however, does more or less prove my initial hypothesis incorrect because my samples which were the most polar proved to be the worst, and quite frankly rather ugly. The mobile phase is always the solvent, and the stationary phase, in this lab, is the paper that was put in the solvent. Identifying the compounds: The ratio of the distance traveled by a component i.
It turned out as said before to be Papermate Blue. Thus, a hypothesis was made to minimize the spreading of the ink samples. The data was then used to calculate the presented R f results. The findings of this experiment in terms of spots colour and Rf results shows that only one of the two unknowns is identified to be arginine. I was also able to identify all the components of the dyes that were made up of more than one.
For the teacher: Make sure to discuss the color change indicator with students. However, there are many other methods that identify ink samples. The major types are the paper chromatography, thin layer, gas chromatography, column chromatography, High performance liquid chromatography, paper chromatography, thin layer chromatography. This solution of 1 part methanol and 2 parts water will have a polarity of 8. While students are conducting the lab, guided by the , walk around the room and ask them questions to keep them engaged and on task. The following chromatographs six and seven were the unknowns that I ran with a polarity of 6. The characteristic was the polarity of the sodium chloride.
Forensic science can use chromatography by separating out physical evidence like ink on questionable documents. One indication that a reaction has not occurred is that no energy change has taken place. Messin' with Mixtures In this activity, students investigate the properties of a heterogeneous mixture, trail mix, as if it were a contaminated soil sample near a construction site. The prefix of Chromo means color and -graphy means writing so it technically means writing in color. The experiment will also help master the technique and be able to analyze the movements made by both unknown mixtures and the known amino acids.
I picked Pilot Easy Touch because of the lighter pink. There are several applications of paper chromatography and other main types of chromatography techniques. So it can be concluded that the first unknown mixture is an arginine amino acid or contain arginine amino acid as a component. It includes teacher instructions for a class demonstration that gives students the chance to compare and contrast the physical characteristics of some simple mixtures and solutions. Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases which are the mobile phases and stationary phase. It still remains unknown, but closer to glycine in identity. We have determined that something with 6.
The analytes are the different dye colors Blue, Green and Red. Push the straight end of the paper clip into the bottom of the rubber stopper. Materials The materials used for this lab are paper, pencil, eraser, filter paper, test tube, rubber stopper, paper clip, metric ruler, black felt-tip pen, and a computer. It is a laboratory test to help discover the components of a substance. We thought since it had all three I. When the unknown chromatographs were ran through, there was not much noticeable difference between the two.
Observe what happens as the liquid travels up the paper. Paper chromatography has proved to be very successful in the analysis of chemical compound and lipid sample in particular. As different inks may appear different under certain wavelengths of light, illuminating an ink with such light can be used to distinguish between them. We saw that the ink would spread too far. The first time I guessed Pilot Easy Touch black, then I guess Bic black. This is very different from absorption. It was made with 3 parts ethanol and 1 part water.
Initially each member of the group, Grei, Ryan, Chris, and I made one. Intermolecular forces is what causes the solvent to separate the different compounds of a mixture Gas Chromatography: it is a common type of chromatography used for separating and analyzing compounds that can be vaporized. I found the green dye is made up of two dyes, blue and yellow. When we ran our standards the 6. For the stationary phase preparation, the required markings are made on the paper for identification and creation of baseline.
This lesson plan introduces students to the properties of mixtures and solutions. Then I measured every 1 cm and put a hash mark. Materials and constituents page 5 The purpose of this lab report is to explain what exactly happened to the labs, the time that the experiment was executed and apply the results and conclusions that were deduced. I would never use that chromatograph if I was working alone because some did not move, and if you look at Pilot Vball Black, it shifted to the right. And, as you can observe, the spreading was very nice and the colors were very bright and differentiated. The… Essays, 404 words Drug addiction is a serious problem in modern society that affects somehow every nation and every family.