Looking under the microscope after letting the stain set will prove to be quite interesting, Results The gram staining lab was a huge success. Simple rules for Making Alliances Studies show that the number of corporate alliances account for nearly 3 of many companies revenue and value. No difficult problems occurred with the identification of B. Thick smears with large clumps of cells can entrap the stain preventing it from being washed. So the real results do not coincide with the planned ones. This test gave a positive result turning a brown color, meaning the gram negative bacteria produced the enzyme casease in order to break down the milk protein casein. Water I I Bunsen Burner Lighter will suffice 11.
Wash with tap water and drain carefully. To avoid the spread and contamination of P. The Maltose test showed positive, but the Glycerol test show incorrect results for negative. It did great job at pulling stains right out of the cloth. Alcohol is added to the sample as a decolouriser, which dehydrates the peptidoglycan layer, shrinking and tightening it. It looked over grown, so the same nutrient agar test was carried out several times. If there is as bubble or splitting in the medium than a gas was produced and a black color signifies hydrogen sulfide production.
This was a negative result for the Voges Proskaer test. Use only special lens paper to clean the lenses. One distinct colony grew and a Gram stain was performed on the isolated colony. This bacterial suspension was transferred to the gray area of the oxidase strip. A stool Gram stain is a laboratory test that uses different stains to detect and identify bacteria in a stool sample. The prelab must be completed prior to the day of the experiment. Within the urea broth all of the essential nutrients that bacteria would need are provided by peptone and glucose and the potassium phosphate is used as a mild buffer that resists alkalinization due to peptone metabolism.
As a result, various laboratory tests were performed to an unknown microbe Unknown 1 found in the water of a nearby pond. Stain the slides as follows: 2 pages, 952 words. The Methyl Red test was positive for the presence of glucose fermentation. Robert Baade is that the costs of a certain project are usually underestimated, and the benefits are overestimated. Pour off excess dye and wash gently in and drain the slide against a paper towel.
Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans have come into contact with. If a smear is too thick one will not be able to see individual cells, their arrangement, or the details of their microstructures. The organic solvent such as acetone or ethanol, extracts the blue dye complex from the lipid-rich, thin walled gram negative bacteria to a greater degree than from the lipid poor, thick walled, gram-positive bacteria. The actual Simmons Citrate Agar has sodium citrate as the carbon source and ammonium phosphate as the nitrogen source. And was decided the bacterial smear is G + or G -. Before bacteria can be stained, a smear of bacteria must be made on a slide and heat fixed.
This test also sees if an organism is and indolent producer. Escherichia coli also known as E. The switch is turned off and the microscope is covered back. For simple staining, only one dye is used. Those that retain the crystal violet are Gram-positive and those that do not retain it are Gram-negative.
Many different types of both gram negative and gram positive bacteria were identified. This proves that unknown number 25 is a fermenter of glucose, lactose and possibly sucrose. The first biochemical test performed was a negative Urea test which left two bacteria. The loops were sterile again to kill excess microbe. They may order it if you have symptoms of a respiratory infection that might be caused by bacteria. The citrate test was performed by streaking a Simmons Citrate slant with an inoculating loop. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures.
Negative stain is particularly useful for determining cell size and arrangement and it can be used to stain cells that are too delicate to be heat-fixed. Stains and dyes are widely used in the scientific field to highlight the structure of the biological specimens, cells, tissues etc. After waiting, it was washed with water. Chemical formula, Chemical formulas, Chemistry 1455 Words 4 Pages The tests used to confirm the fermentation were a gas production test, pH test, Gram stain, and turbidity test. Once the test tube is retrieved, a steak for isolation will be completed in order to produce.